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ADA Determination Assay

Application             Determination of Adenosine Deaminase Activity in serum, Plasma and                                                Biological  Fluids

Principle                    ADA–ENZYMATIC REACTION

                                   Intensity of blue coloured indophenol complex is directly proportional to                                              amount of ADA present in sample.

Measurement                Blue Indophenol Complex (End Product) can be read on                                                                      Spectrophotometer  with filter at 570-630 nm at 37°C or Colorimeter with                                             yellow or red filter.

Reagent System         a) L1 - ADA- MTB- Buffer Reagent, ready to use. 

                                    b) L2 - ADA- MTB- Adenosine reagent ready to use. 

                                    c) L3 - ADA- MTB- Phenol Reagent 

                                    d) L4- ADA- MTB- Hypochlorite Reagent 

                                     e) S- ADA- MTB - Calibrator (Ready to use)

Storage / Stability              2-8°C, 15 months

In Use Stability                 Reconstituted

                                           Working reagent: both Phenol Reagent (L3) & Hypochlorite (L4) need                                               to be diluted 1:5 with distilled water.2-8°C, at least 6 months when                                                     stored in  tightly capped bottle

                                          Calibrator: Ready to use2-8°C, 15 months provided it is not                                                                contaminated.

Presentation                                                                              Pack                                         

ADA-MTB Kit contents                                                              15 Tests

 a) L1 - ADA- MTB- Buffer Reagent, ready to use.   

 b) L2 - ADA- MTB- Adenosine reagent ready to use.    

c) L3 - ADA- MTB- Phenol Reagent   

 d) L4- ADA- MTB- Hypochlorite Reagent    

e) S- ADA- MTB - Calibrator (Lyophilized)

Use for Hospital Lab

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